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A lab engineered strain of E.coli that can sustainably convert used plastic waste into vanillin – the key ingredient in vanilla flavouring – has been discovered at the University of Edinburgh. The findings, published recently in the journal Green Chemistry, have “major implications for the field of plastic sustainability,” first author and Biotechnology and Biological Sciences Research Council (BBSRC) Discovery fellow, Joanna Sadler, said in an accompanying press statement.It is the first example of biological upcycling of waste polyethylene terephthalate (PET) plastic into a single “value-added” small molecule.Vanillin is the primary component of extracted vanilla beans which provides the taste and smell of vanilla in flavourings. Beside food, it is widely used in cosmetics, pharmaceuticals, cleaning products and herbicides. Global demand for vanillin exceeded 37,000 tonnes in 2018 and is expected to top 59,000 tonnes by 2025, with revenues of $734 million, said the researchers, citing Grand View Research figures. The discovery could provide a “tasty solution” to the world’s mounting plastic crisis, as scientists race to develop new and improved recycling processes for the masses of PET used globally, including in food and drinks packaging, according to the press statement. The plastic is produced from nferrous fumarate 210mg nhson-renewable materials such as oil and gas, with around 50 million tonnes of PET waste produced annually, leading to plastic pollution and serious environmental impact. “There is an urgent need to develop ferrous fumarate 305mg twice a daytechnologies to valorise post-consumer PET waste to tackle plastic pollution and move towards a circular economy,” the researchers stressed. “Whilst PET degradation and recycling technologies have been reported, examples focus on repurposing the resultant monomers to produce more PET or other second-generation materials.”Economic incentives are also associated with the discovery since plastics lose 95% of their material value after a single use, the researchers said, “leading to an estimated $110 billion loss to the global economy per annum”. Enzymes have already beferrous fumarate 140mg 5ml oral solutionen developed to breakdown PET polymer into terephthalic acid (TA). Engineering a novel biosynthetic pathway in the laboratory bacterium E.coli, the researchers developed a process to convert TA directly from “post-consumer plastic waste” into vanillin in a “one-pot process”. After process optimisation, they were able to achieve 79% TA to vanillin conversion. This conversion rate was a 1ferrous fumarate 210mg tablets boots57-fold improvement over pre-optimisation experiments, the researchers added, stressing that the key to this high percentage was “biotransformation temperature, cell permeabilisation and use of [in situ product removal] to increase flux towards vanillin”.The reaction is “mild, uses a whole-cell catalyst produced from renewable feedstocks and occurs under ambient conditions (room temperature, pH5.5–7), in aqueous media,omeprazole and ferrous sulfate requires no additional cofactors or reagents and generates no hazardous waste,” the researchers said. In future, studies will focus on intensifying the process through further strain engineering, process optimisation and extension of the pathway to other metabolites, they added. “Fundamentally, this work substantiates the philosophy that post-consumer plastic may be viewed not as a waste product, but rather as a carbon resource and feedstock to produce high value and industrially relevant materials and small molecules,” the researchers concluded.Use of microbes to turn environmentally-harmful waste plastics into an important commodity and platform molecule with broad applications in cosmetics and food is a “beautiful demonstration of green chemistry,” Dr Ellis Crawford, publishing editor at the Royal Society of Chemistry said.
Bacteria can be used to convert plastic waste into vanilla flavouring
